Journal: Neural Regeneration Research

Article Title: Effect of type-2 astrocytes on the viability of dorsal root ganglion neurons and length of neuronal processes

doi: 10.4103/1673-5374.125339

Figure Lengend Snippet: Immunofluorescence images of oligodendrocyte precursor cells from the spinal cord of adult rats and differentiated oligodendrocytes. (A) Primary cultured oligodendrocyte precursor cells were double-stained with A2B5 (green fluorescence) and Hoechst 3442. (B) Oligodendrocyte precursor cells (passage 3) were double-stained with A2B5 (green fluorescence) and Hoechst 3442. (C) Oligodendrocytes appearing at 3 days of oligodendrocyte precursor cell differentiation were double-stained with O1 (red fluorescence) and Hoechst 3442. Blue fluorescence: Hoechst 3442-labeled nuclei. Scale bars: 50 μm.

Article Snippet: Secondary antibodies were Texas Red- or FITC-labeled donkey anti-mouse IgM (mouse anti-A2B5, O1 or O4) (1:100; Jackson Immuno Research), Rhodamine-labeled donkey anti-rabbit IgG (rabbit anti-NF-M and rabbit anti-glial fibrillary acidic protein) (1:200; Jackson Immuno Research), Rhodamine- or FITC-labeled donkey anti-chicken IgG (chicken anti-glial fibrillary acidic protein) (1:200; Jackson Immuno Research) at room temperature in the dark for 1 hour.

Techniques: Immunofluorescence, Cell Culture, Staining, Fluorescence, Cell Differentiation, Labeling

Journal: Neural Regeneration Research

Article Title: Effect of type-2 astrocytes on the viability of dorsal root ganglion neurons and length of neuronal processes

doi: 10.4103/1673-5374.125339

Figure Lengend Snippet: Immunofluorescence images of oligodendrocyte precursor cells after differentiation induced by bone morphogenetic protein-4. Blue fluorescence: Hoechst 3442-labeled nuclei. (A–D) Cells were triple-stained with O1 (green fluorescence), glial fibrillary acidic protein (GFAP) (red fluorescence) and Hoechst 3442 at 1, 3, 5 and 7 days after differentiation of oligodendrocyte precursor cells. (E) Cells were triple-stained with A2B5 (red fluorescence), GFAP (green fluorescence) and Hoechst 3442 at 5 days after differentiation of glial-restricted precursor cells induced by bone morphogenetic protein-4. Scale bars: 50 μm.

Article Snippet: Secondary antibodies were Texas Red- or FITC-labeled donkey anti-mouse IgM (mouse anti-A2B5, O1 or O4) (1:100; Jackson Immuno Research), Rhodamine-labeled donkey anti-rabbit IgG (rabbit anti-NF-M and rabbit anti-glial fibrillary acidic protein) (1:200; Jackson Immuno Research), Rhodamine- or FITC-labeled donkey anti-chicken IgG (chicken anti-glial fibrillary acidic protein) (1:200; Jackson Immuno Research) at room temperature in the dark for 1 hour.

Techniques: Immunofluorescence, Fluorescence, Labeling, Staining